Development of a recombinant protein based dot-blot for the diagnosis of canine leptospirosis
نویسندگان
چکیده
منابع مشابه
Development of a recombinant protein based dot-blot for the diagnosis of canine leptospirosis
Background Leptospirosis is a globally disseminated zoonosis, recognized as a re-emergent neglected disease [1] and the causative pathogens are spirochetes from genus Leptospira [2]. In Brazil, more than 100.00 leptospirosis cases are reported annually, with outbreaks emerging especially during floods [3]. The microscopic agglutination test (MAT) is considered the standard serological test for ...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملDevelopment of a recombinant protein based ELISA for the diagnosis of canine leptospirosis
Background Leptospirosis is an emergent zoonotic disease caused by pathogenic Leptospira spp., and humans are regarded as an accidental or dead-end host [1]. The global incidence of severe human leptospirosis was estimated to be approximately 500.000 cases worldwide, although this is likely an underestimate due to the lack of a reliable diagnostic test. Mortality ranges from 10 to > 50% for Wei...
متن کاملdevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...
متن کاملRecombinant multiepitope protein for diagnosis of leptospirosis.
Leptospirosis is an emerging infectious disease and is considered to be the most widespread zoonotic disease in the world. It can be misdiagnosed because manifestations of this febrile disease vary from mild flu-like symptoms to severe illness involving vital organs such as the liver and lungs. Therefore, accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections pr...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2014
ISSN: 1753-6561
DOI: 10.1186/1753-6561-8-s4-p144